K-4214 - ExiPrep Plus Bacteria Genomic DNA Kit
ExiPrep™ Plus Bacteria Genomic DNA utilizes a resuspension buffer that efficiently disrupts cells and proteins for efficient extraction of genomic DNA. Gram (+) bacteria needs specific pre-treatment before the re-suspension step using Lyticase or Lysozyme (not included). The binding buffer and silica magnetic particles will bind genomic DNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the genomic DNA-bound silica magnetic particles to remain within the wells while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the genomic DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified genomic DNA.
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