TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O. Efficiency of PCR products with “A” is equal to EasyTaq® DNA polymerase. It is more suitable for high fidelity TA cloning.
• TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.
Applications
• Complex templates
• TA cloning
Storage
at -20°C for two years
Shipping
Dry ice (-70°C)
Product Contents
Component |
AS122-01/21 |
AS122-02/22 |
2×TransTaq®-T PCR SuperMix (-dye)/(+dye) |
1 ml |
5×1 ml |
Nuclease-free Water |
1 ml |
5 ml |