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Bio Reagents & Consumables
Cell Detection
Caspase-9 Assay Kit (Colorimetric)
Caspase-9 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-9.
Brand: Abbkine
Not Available
Free delivery within Singapore only
Specifications
| Applications notes | Caspase 9 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-LEHD-pNA (acetyl-Leu-Glu-His-Asp p-nitroanilide) by caspase 9, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. |
Product Properties
| Kit components | • Cell Lysis Buffer • Reaction Buffer (2×) • Ac-LEHD-pNA (4mM) • pNA(10mM) • DTT (100×) |
| Features & Benefits | • Simple and optimized procedure. • Fast and convenient. • Detect early/middle stages of apoptosis. |
| Storage instructions | Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-9 (also known as ICE-LAP6 and Mch6) is a member of the caspase-family of cysteine proteases. Caspase-9 is activated by recruitment and dimerization within the Apaf-1 apoptosome. Once recruited, caspase-9 undergoes proteolytic cleavage at Asp315 to yield 35-kDa and 10-kDa fragments. Unlike other caspases, this cleavage event is not required for the catalytic activity of caspase-9. As an initiator caspase, this protease initiates a caspase signaling cascade that results in apoptosis. |
Specification :
| Specifications | Kindly refer to the "Overview" tab. |
