Cell Counting and Viability Assays

AO/PI Viability Assay for CellDrop FL

The Acridine Orange (AO) / Propidium Iodide (PI) assay is used for cell counting and viability. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. All live, nucleated cells fluoresce green due to AO, while dead, nucleated cells are stained with both AO and PI and fluoresce red.

CAT# CD-AO-PI-1.5

Components  1.5 mL @ mix of 5 ug/mL AO+ 100 ug/mL PI in PBS

300 Assays

Acridine Orange

The Acridine Orange (AO) Dye is used for total cell counting. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells.

CAT# CD-AO-1.5

Components 1.5 mL @ 10 ug/mL in PBS

300 Assays

Propidium Iodide

The Propidium Iodide (PI) Dye is used for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells.

CAT# CD-PI-1.5

Components 1.5 mL @ 100 ug/mL in PBS

300 Assays

Yeast Viability Assay Kit

The Fluorescein Diacetate (FDA) and Propidium Iodide (PI) assay is used for yeast cell viability testing. FDA is a cell viability probe that is membrane-permeable to live yeast cells. Inside a live cell, FDA is cleaved to give fluorescein by the action of intracellular esterases. Propidium Iodide dye for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. Live cells are reported by FDA in the green channel, dead cells by PI in the red channel.

CAT# KIT-CD-YEAST-1.5

Components 1.5 mL PI, 1.0 mL FDA, 20 mL PBS buffer

300 Assays

Trypan Blue

The Trypan Blue (TB) dye is used for brightfield viability assessment. TB is an azo dye that is excluded from cells with intact cell membranes, only staining non-viable cells. The trypan blue assay is used to assess the number of viable cells present in a cell suspension.

CAT# CD-TRY-1.5

Components 1.5 mL @ 0.4% in PBS

300 Assays

Brightfield Beads

  Brightfield beads for CellDrop FL and CellDrop BF.

CAT# CD-BRB-0.5

Components 0.5 mL @ 1×10⁶

50 Samples

Fluorescence Beads

  Fluorescent beads for CellDrop FL.

CAT# CD-FLB-0.5

Components 0.5 mL green / red mix @ 1×10⁶

50 Samples

DeNovix 20uL Pipette

  Pipette for CellDrop.

CAT# PIPETTE-M-20uL

Capacity 2.0 – 20.0 uL

Ergonomic design