CytoSinct™ - Cell Isolation and Activation Solution

Overview

Efficient and gentle cell isolation is crucial for researching specific cell populations and developing life-saving cell and gene therapy products. Purity, recovery, and viability are key characteristics post-isolation.

GenScript excels in developing magnetic bead products for biomolecule separation. CytoSinct™ magnetic beads use immunomagnetic cell separation technology, combining antibody-based purification specificity with nanoparticle gentleness.

The CytoSinct™ manual cell isolation platform includes antigen-specific paramagnetic nanobeads, a column, and a magnetic separator. These nanometer-sized, biodegradable matrix-coated beads are non-toxic, user-friendly, ensuring highly efficient cell isolation. CytoSinct™ columns amplify the magnetic field for effective separation with minimal labeling. Isolated cells are compatible with downstream applications like cell culture, activation, expansion, flow cytometry, and translational research.

With the flexibility and specificity of CytoSinct™, you can use various starting materials for enriching or depleting desired cell types. Each use guarantees high-purity, high-recovery isolated samples.

Product Highlights

• Highly efficient
• Biodegradable matrix
• Nontoxic
• Compatible
• Easy to use
• Sterilized
• Paramagnetic

Workflow

Highly Efficient CD4+CD8+T Cell Isolation from PBMCs

Purity, recovery, and viability are crucial characteristics of cells post-isolation. CytoSinct™ Nanobeads, being nanometer-sized, enable gentle and efficient isolation of target cells. This ensures that your post-isolation cells are suitable for a variety of downstream applications.

PBMCs were isolated using CytoSinct™ CD4 Nanobeads, human (RUO) (Cat# L00863) and CytoSinct™ CD8 Nanobeads, human (RUO) (Cat# L00864), theresults shows high purity(A,B), high recovery(C) and high viability(D).
 

Comparing to the similar products from the competitor, GenScript CytoSinct™ Nanobeads can provide the target cells with high purity and recovery rate.

Subpopulation phoenotype of post-isolation cells is important for functional and phenotypic studies. Non-T subpopulations like monocyte and NK cells could impact the efficacy of T-cells or therapeutic CAR-T and TCR-T generated from the enrichment. GenScript CytoSinctTM NanoBeads for CD4 and CD8 T-cell isolation efficiently minimize non-T composition in the enrichment population.

Highly Efficient T Cell Isolation from Apheresis

CD4+&CD8+ or CD3+ T cell isolation are both commonly used for the relevant T cell studies. GenScript can provide high quality of both RUO and GMP grade CytoSinct™ Nanobeads, which can support your studies from bench-side to bed-side.

Representative data comparing T cells isolation from leukopheresis samples using GenScript CytoSinctTM Nanobeads and competitor products.
A. Shows comparison of pre and post isolation data, purity, recovery, and viability for co-isolation of CD4 and CD8 T cell from apheresis using CytoSinctTM CD4 + CytoSinctTM CD8 and competitor reagent.
B. Shows comparison of pre and post isolation data, purity, recovery, and viability. CD3 T cell from apheresis using CytoSinctTM CD3 and competitor reagent.

Isolated T cells using CytoSinct™ Nanobeads has a high purity and recovery rate.

Enceed™ — World Leading T Cell Activation Reagent

GenScript Enceed™ T cell activation uses CD3 and CD28 as the activation marker for in vitro activation and expansion of human T cells. Comparing to the similar products from the competitor, activated T cells using Enceed™ can obtain higher expansion folds and larger total amount of TSCM+TCM, which is greatly beneficial for the downstream application.

(A) Isolated CD4+CD8+T cells were activated using the Enceed™ T cell activation reagent from GenScript, comparing with the similar activation product from the competitor. After the activation, the cell samples were cultured and expanded for 48 hours, and the CD25+ & CD69+ expression were detected by flowcytometry and shown.

(B) To show the expansion status after the activation, isolated T cells were stained with CFSE before and 7 days after the activation.

(C) The expansion folds of the activated T cells were shown along the course of 17 days with the comparison of the activated T cells using competitor's product.

(D) The memorial phenotypes of the activated T cells were monitored and shown on Day 12 and Day 17 after the activation.

CytoSinct™ Column and Magnetic Separator

CytoSinct™ Column 

• Anti-clogging property: Innovative structure
• Compatibility: compatible with beads from other vendors
• High performance: High purity, recovery and viability cells after separation

CytoSinct™ Magnetic Separator 

• High Recovery & Fast Isolation
• Flexible Throughput(single/multi-channel)
• Easy to operate

CytoSinctTM 1000 Automated Cell Isolation Instrument

Representative data of Cell Isolation with CytoSinct™ 1000 system

• GenScript CytoSinct™ 1000 system can achieve a high purity, recovery rate and viability of the target cells.
• Higher cell recovery rate is observed, since CytoSinct™ 1000 instrument has a relevant higher elution efficiency.
• The closed and sterilized CytoSinct™ 1000 Tubing Set Products are optimized for different applications.

Learn more

Cell Therapy Product List

Cell Isolation Nanobeads

RUO grade Nanobeads

GMP grade Nanobeads

Enceed™ Activation Reagents

RUO grade Activation Reagent

GMP grade Activation Reagent

Manual Separators

CytoSinct™1000 (Clinical Grade System)