EasyPure® EndoFree Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA from ≤500 ml(LB) of bacterial culture. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the operation. Plasmid purification process include special endotoxin removal solution. Endotoxin level can reach as low as <0.1 EU/µg. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.
Features
• Visualized operation: solution LB (blue) indicates whether the lysis and neutralization are complete through the change of color.
• Fast: the whole procedure can be performed in one hour.
• High purity: high-purity, endotoxin-free (<O.lEU/µg) transfection-grade plasmid DNA can be prepared.
• High yield (up to 4 mg nucleic acid load in the purification column).
Storage
At room temperature (15-25 °C)
Shipping
At room temperature (15-25 °C)
Product Composition
| Component |
EM123-01 (10 rxns) |
| Resuspension Buffer (RB) |
120 ml |
| Lysis Buffer (LB, Blue) |
120 ml |
| Neutralization Buffer 1 (NB1) |
120 ml |
| Activation Buffer (AB) |
55 ml |
| Endotoxin Removal Buffer (ER) |
36 ml |
| Wash Buffer (WB) |
25 ml |
| Elution Bufffer (EB) |
25 ml |
| RNase A (10 mg/ml) |
1.2 ml |
| Maxi-Plasmid Spin Column with Collection Tube |
10 each |
| Push Filter |
10 each |
| 50 ml Collection Tube |
10 each |
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