EasyPure® Genomic DNA Kit provides a simple and convenient way to efficiently isolate high-quality genomic DNA from a variety of materials (mammalian cells, tissues, mouse tails, E. coli and yeast). Cells and tissues are enzymatically lysed. DNA is specifically bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot, etc.
• Fast extraction, high yield (up to 15 μg).
• High integrity of cDNA ensured by mild lysis conditions without physical disruption, thus reducing damage to gDNA during cell lysis process.
• High purity enabled by spin column which can efficiently and specifically bind to DNA and removs protein, salts, lipids or other contaminants.
Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) in a dry place for one year.
Shipping
RNase A on dry ice (-70 °C); Others at room temperature.
Product Contents
Component |
EE101-01 (50 rxns) |
EE101-02 (200 rxns) |
EE101-11 (50 rxns) |
EE101-12 (200 rxns) |
Lysis Buffer 2 (LB2) |
6 ml |
24 ml |
6 ml |
24 ml |
Binding Buffer 2 (BB2) |
28 ml |
110 ml |
28 ml |
110 ml |
Clean Buffer 2 (CB2) |
15 ml |
60 ml |
15 ml |
60 ml |
Wash Buffer 2 (WB2) |
12 ml |
2×22 ml |
12 ml |
2×22 ml |
Elution Buffer (EB) |
25 ml |
80 ml |
25 ml |
80 ml |
RNase A (20 mg/ml) |
1 ml |
4×1 ml |
0 |
0 |
Proteinase K (20 mg/ml) |
1 ml |
4×1 ml |
1 ml |
4×1 ml |
Genomic Spin Column with Collection Tubes |
50 each |
200 each |
50 each |
200 each |