EasyPure® Plasmid MiniPrep Kit uses a modified alkaline lysis method to isolate high-quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation and DNA sequencing.
• Simple and fast: the whole procedure can be performed in 20 minutes.
• High yield: DNA yield up to 40 μg.
Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) in a dry place for one year.
Shipping
RNase A on dry ice (-70°C); others at room temperature.
Product Contents
Component |
EM101-01 (50 rxns) |
EM101-02 (200 rxns) |
Resuspension Buffer (RB) |
15 ml |
60 ml |
Lysis Buffer (LB) |
15 ml |
60 ml |
Neutralization Buffer (NB) |
20 ml |
80 ml |
Wash Buffer (WB) |
10 ml |
2×20 ml |
Elution Buffer (EB) |
5 ml |
10 ml |
RNase A (10 mg/ml) |
150 μl |
600 μl |
Mini-Plasmid Spin Column with Collection Tubes |
50 each |
2×100 each |