The kit is designed for the isolation of RNA from various fresh and dried plant samples, including plant samples rich in polysaccharides and polyphenols. The kit is based on unique extraction system and membrane technology to specifically remove secondary metabolites such as polysaccharides, polyphenols, and lipids in samples, without the need for toxic reagents such as phenol and chloroform. Purified RNA with high quality and good stability can be used for RT-PCR, qRT-PCR, Northern blot, RACE, library construction, etc.
Storage: at room temperature (15-25°C) in a dry place for one year; DNase I at -20°C for one year.
shipping:at room temperature
Product Composition
Component |
ER302-01 (50 rxns) |
ER302-02 (200 rxns) |
Lysis Buffer 52 (LB52) |
40 ml |
160 ml |
Precipitation Buffer (PB52) |
10 ml |
40 ml |
DNase 1 (3 units/µl) |
1500 U |
6000 U |
DNase 1 Reaction Buffer |
4 x 1 ml |
15 ml |
Clean Buffer 52 (CB 52) |
20 ml |
80 ml |
Wash Buffer 52 (WB 52) |
12 ml |
2 x 22 ml |
RNase-free Water |
10 ml |
20 ml |
Filtration Columns with Collection Tubes |
50 |
200 |
RNA Spin Columns with Collection Tubes II |
50 |
200 |