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Bio Reagents & Consumables
PCR Enzymes
EasyTaq® DNA Polymerase for PAGE
EasyTaq® DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 3 kb.
Applications
Short fragment PCR
Storage
at -20°C for two years
Shipping
Dry ice (-70°C)
Product Contents
| Component | AP112-01/11 | AP112-02/12 |
| EasyTaq® DNA Polymerase for PAGE | 2500 U | 2500 U×4 |
| 10×EasyTaq® Buffer for PAGE | 1.2 ml ×5 | 1.2 ml×20 |
| 2.5 mM dNTPs | - / 800 μl×5 | - / 800 μl×20 |
| 6×DNA Loading Buffer | 1 ml | 1 ml ×4 |
| Product Contents | Kindly refer to the "Overview" tab. |
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