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Bio Reagents & Consumables
ELISA (Enzyme-linked Immunosorbent Assay)
ELK2100 Human Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit
Human ELISA Kit
Brand: ELK Biotechnology
Available
Free delivery within Singapore only
Overview
| Product name: | Human Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42 |
| Assay Type: | Sandwich |
| Sensitivity: | 5.31 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Neuro science; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.161 | 2.078 |
| 500.00 | 1.641 | 1.558 |
| 250.00 | 1.234 | 1.151 |
| 125.00 | 0.969 | 0.886 |
| 62.50 | 0.543 | 0.460 |
| 31.25 | 0.351 | 0.268 |
| 15.63 | 0.291 | 0.208 |
| 0.00 | 0.083 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-42 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 88-102% | 95% |
| EDTA plasma(n=5) | 90-105% | 97% |
| Heparin plasma(n=5) | 80-92% | 86% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-94% | 87-96% | 79-95% | 95-102% |
| EDTA plasma(n=5) | 85-92% | 79-96% | 87-103% | 90-99% |
| Heparin plasma(n=5) | 88-97% | 96-105% | 91-103% | 89-97% |
Specification :
| Details | Kindly refer to the "Overview" tab. |
