Endpoint Chromogenic Kit EC64405

Overview

Bioendo EC Endotoxin Test Kits provide a kind of measurement for quantitative endotoxin testing solution. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored product with maximum absorbance at 405nm. For End-point Chromogenic Endotoxin Test Kit without Diazo Coupling, a regular spectrophotometer or microplate reader is required to read the absorbance at 405nm. The regular spectrophotometer with the function of kinetic experiment, the accuracy of temperature control and 405nm filter, then use that spectrophotometer can read the quantitative endotoxin result of EC kit.

Product information

End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is conducted by adding a colorless artificial peptide substrate solution into mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. If test sample contain endotoxin, a yellow color will develop in the 96 well microplate. Its absorbance (λmax = 405nm) is related to the endotoxin concentration. Endotoxin concentration of test sample could be calculated based on a standard curve.

Product parameter

Sensitivity Range: 0.01-0.1 EU/ml, matching assay time is about 46 minutes

                              0.1-1 EU/ml, matching assay time is about 16 minutes. 

Product features and application 

End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into the mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. Yellow color develops if test sample contains endotoxin. Then use regular spectrophotometer or plate reader to read the absorbance at 405nm. End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is suitable for all kinds of biomedical samples such as protein, vaccine, plasmid, DNA, RNA endotoxin assay. 

Note

Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.

Product condition

The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin. The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.

Does the End point endotoxin test kit need sophisticated microplate reader?

Bioendo EC64405 and EC64405S kit analysis the quantitative endotoxin results through regular microplate reader. 

End point chromogenic endotoxin test kit series:

• Endotoxin Free Tube
• Pyrogen Free Tips
• Pyrogen Free Microplates
• Regular Microplate reader
• Matched Control standard endotoxin (CSE10V)
• BET water use for reconstitution