Genome Editor Electroporator

Overview

Genome Editor^TM is a simple and easy-to-use electroporator suitable for zygote genome editing using CRISPR/Cas9 system.

Application

High throughput and effcient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote.

Fgf10 gene knockout mouse by Cas9 mRNA/gRNA electroporation

Fgf10 homozygous mutant embryo have an easily detectable limbless phonotype. Electroporation of Cas9 mRNA (Cas9 Protein) and gRNA enables genome editing of Fgf10.

Electrodes for zygote genome editing

Platinum plate electrode on tempered glass, LF501PT1-10, can hold up to ~40 mouse zygotes in a 5 μL electroporation buffer and enables efficient and high throughput zygote genome editing using CRISPR/Cas9 by electroporation.

Electroporation vs Microinjection

More zygotes can be treated at one time by electroporation than microinjection.

Viability of treated zygotes are much higher in electroporation than in microinjection.

No special skills are required for electroporation compared to microinjection, which requires injection technique that is time-consuming to acquire.

Electroporation is, therefore, the ideal method for high throughput mouse zygote genome editing by CRISPR/Cas9 system.

Specifications