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Life Science
Electroporator
Genome Editor Plus Electroporator
Overview
This is ONE for all the early embryo experiment!
The function for oocyte activation and tetraploid formation is added to Genome Editor, which has been popular since it's released. This enables to do every experiments related to early embryos with only one unit of this Genome Editor plus.
Features
- The function to output AC voltage is added to Genome Editor, which enhanced zygote genome editing researches.
- Two mode: AC + DC pulses for oocyte activation and tetraploid formation; and DC pulses for zygote genome editing.
- Easy-to-use operation to program the parameter by touch panel
- All the data of executed pulse output parameters can be exported through USB memory.
- All the electrodes for CUY electroporator series and LF electrofusion series are connectable.
Application
High throughput and effcient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote
Fgf10 homozygous mutant embryo have an easily detectable limbless phonotype. Electroporation of Cas9 mRNA (Cas9 Protein) and gRNA enables genome edit of Fgf10.
Electrode for zygote electroporation
LF501PT1-10 is applied for zygote genome editing, newly developed for zygoe electroporation.
Comparison between electroporation and microinjection
|
|
Electroporation |
Microinjection |
|---|---|---|
|
Pre-operation |
Not necessary |
Preparation of injection and hold pippettes, etc.
|
|
Required time |
~5 minutes |
> 2 hours |
|
Viability (after E15) |
40-80% |
10-50% |
|
Efficiency |
Same as microinjection |
Depends on the sequence |
|
Required skills |
Not necessary |
Maniipularion of single zygotes |
|
Required amount of Cas9 mRNA |
500 - 2,000 ng |
50 - 500 ng |
More zygotes can be treated at one time by electroporation than microinjection.
Viability of treated zygotes are much higher in electroporation than in microinjection.
No special skills are required for electroporation compared to microinjection, which requires injection technique that is time-consuming to acquire.
Electroporation is , therefore, the ideal method for high throughput mouse zygote genome editing by CRISPR/Cas9 system.
Specifications
GenomeEdit Mode
| Voltage | 1 - 200 V / 1 V resolution |
|---|---|
| Max current | 1 A (1000 mA) |
| Pulse length (Pon) | 0.10 - 1000 ms 0.10 - 9.99 ms (0.01 ms resolution) 10.0 - 99.9 ms (0.1 ms resolusion) 100 - 1000 ms (1 ms resolution) |
| Pulse interval | 1 - 1000 ms 1.0 - 9.99 ms (0.01 ms resolution) 10.0 - 99.9 ms (0.1 ms resolution) 100 - 1000 ms (1 ms resolution) |
| Output pattern | Single polar pulse / Bi-polar pulse |
| Pulse number | 1 - 1000 (for single polar pulse (Pd(+)) 1 - 500 (for Bi-polar pulse (Pd(+/-) or Pd(Alt)) |
| Impedance measurement range | Max 4 kΩ |
Fusion (Activation) Mode
AC Pulse
| Voltage | 1.0 - 20.0 V (0.1 V resolution) | ||
|---|---|---|---|
| Output time | 0,1 - 99.9 sec (0.1 sec resolution) | ||
|
Frequency |
1 MHz | ||
DC Pulse
| Pulse Shape | Square pulse |
|---|---|
| Voltage | 1 - 200 V (1 V resolusion) |
| Max current output | 1 A (1,000 mA) |
| Pulse length | 1 - 999 μ sec (1 μ sec resolution) |
| Pulse interval | 0.01 - 9.99 sec (0.01 sec resolution) |
| Pulse number | 1 - 1,000 pulses |
Other
| Dimensions | W 240 mm X L 380 mm X H 190 mm (w/o rubber stand, umbo) |
|---|---|
| Weight | 6 kg |
|
Power |
100 - 115 V or 220 V, 50/60 Hz |
| Power Consumption | 260 VA |
| Specifications | Kindly refer to the "Overview" tab. |
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