GFP mRNA

Overview

GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It can be used as control of transfection efficiency. 

OZ Biosciences mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.

GFP mRNAs:

• #MRNA15 does not bear any additional nucleotide modifications.
• #MRNA11 is modified with 5-methoxyuridine (5moU) to reduce innate immune response.
• #MRNA22 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response.
• #MRNA15c and #MRNA11c are labelled with Cy5 (Sulfo-Cyanine5) by replacing 20 % of U or moU by UTP-Cy5.

We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.

Size: 20 µg, 100µg and 1 mg

Storage: -80°C

mRNAs ARE SHIPPED WITH DRY ICE

Applications 

GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Results

Figure 1: Jurkat T cells, Raw264.7, DC2.4, primary human Adipose Stem Cells, pri-mary human Mesenchymal Stem Cells and Mouse Embryonic Fibroblasts were transfected using RmesFect transfection reagent and mRNA-GFP from OZ Biosciences.