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Analytical Science
High Performance Chromatographs (HPLC) / Amino Acid Analyzers (AAA)
High-Performance Liquid Chromatograph Chromaster® 5610 MS Detector
Easy Acquisition of Mass Information!
The Hitachi Chromaster 5610 mass detector is designed for HPLC applications, that may not require a large-scale mass spectrometer.
- Improved chromatogram reliability
- Easy acquisition of mass information
- No need for special installation environment
- Easily maintained
*:"Chromaster" is a registered trademark of Hitachi High-Tech Corporation in Germany and France.
Features
Compact tabletop design - Ideal footprint for any HPLC laboratory
- With its space-saving compact design, its footprint is equivalent to that of a HPLC system.
- The N2 gas usage has been reduced (maximum flow rate of 3.4 L/min).
The system can be operated with a N2 gas cylinder. - Can be connected to an existing HPLC (inquire about the compatibility).
Site Requirements
Usability Equivalent to Optical HPLC Detectors
- Instrument and method settings
Ease of use is achieved by eliminating complicated MS settings for those new to mass analysis.
The Chromaster 5610 is equipped with an auto-tuning function to provide optimized instrument conditions to help HPLC users who are unfamiliar with the operation of mass spectrometers. - Measurement Results
Functions such as the contour display allow the verification of an overall chromatographic elution pattern. The user interface resembles that of a PDA detector so operations are as simple as using HPLC detectors.
Measurement result by scanning mode
Maintainability Equivalent to Optical HPLC Detectors
- Maintenance
When reduced sensitivity or contamination is suspected, the atmospheric-pressure ion filter can be removed for easy cleaning.
As the vacuum pump can keep running while the filter is being cleaned, the measurements can be started immediately after the maintenance.
Procedure for removal of the atmospheric filter
The atmospheric ion filter can be removed in 3 steps, and it can be reused after ultrasonic cleaning.
- Instrument and method settings
Ease of use is achieved by eliminating complicated MS settings for those new to mass analysis.
The Chromaster 5610 is equipped with an auto-tuning function to provide optimized instrument conditions to help HPLC users who are unfamiliar with the operation of mass spectrometers. - Measurement Results
Functions such as the contour display allow the verification of an overall chromatographic elution pattern. The user interface resembles that of a PDA detector so operations are as simple as using HPLC detectors.
Measurement result by scanning mode
Maintainability Equivalent to Optical HPLC Detectors
- Maintenance
When reduced sensitivity or contamination is suspected, the atmospheric-pressure ion filter can be removed for easy cleaning.
As the vacuum pump can keep running while the filter is being cleaned, the measurements can be started immediately after the maintenance.
Procedure for removal of the atmospheric filter
The atmospheric ion filter can be removed in 3 steps, and it can be reused after ultrasonic cleaning.
Specifications
| Items | Description |
|---|---|
| Measurement mass range (m/z) | 20 - 1,000 |
| Ion source | Electrospray ionization (ESI) |
| Dimensions | 440(W) × 600(D) × 430(H) mm |
| N2 gas usage | Max flow rate 3.4 L/min, Pressure 300 ± 20 kPa |
Data
FIA*¹ Measurement (with autosampler and pump)
As the mobile phase is delivered by a pump, a small volume of sample is injected and measured by “Flow Injection Analysis Method”. By connecting the HPLC to an autosampler, the measurement can be automated and it can be used for the confirmation of mass information in each sample.
*1Flow injection analysis
Monitoring of Mass Information for Compounds
Confirmation of the mass information for a compound is easier,
By simply setting the sample(s) to be analyzed in the autosampler, the mass information can be obtained. Monitoring a sequence analysis is also available.
*This sample was provided by the Department of Pharmaceutical Molecular Design, Meiji Pharmaceutical University.
*FIA sequence analysis such as the monitoring introduced here, the UI pad or GUI controller is required to control the HPLC module.
LC-MS Measurement
During a HPLC experiment, by adding the mass spectral information collected by the MS detector to the measurement data obtained by the UV (or similar) detector, the reliability of the identification result is enhanced. In addition, the SIM (selected ion monitoring) measurement improves the selectivity for the target compound. The method is particularly useful for the measurement of non-UV-absorbing samples.
Measurement of bacterial culture
By connecting the diode array detector and MS detector in series, the UV spectrum and mass spectrum can be simultaneously obtained.
With this system configuration, a large amount of sample information can be obtained. In addition, the measurement result obtained by the scanning mode of the MS detector is shown in the form of a contour display, which is commonly used for displaying data from diode array detectors. Therefore, the identity of the detected ion can be more accurately determined.
Measurement Result by Diode Array Detector
Measurement Result by 5610 MS Detector
*The data introduced here was provided by the Department of Microbial Chemistry, Kitasato University.
*These data are an example of measurement; the individual values cannot be guaranteed.
Display of Measurement Result
