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Bio Reagents & Consumables
Cell Detection
Live and Dead Cell Double Staining Kit
Specifications
| Applications notes | Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and NucleiDye, a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells. |
Product Properties
| Kit components | • LiveDye • NucleiDye • Assay Buffer (10X) |
| Features & Benefits | • Suitable for mammalian cells staining. • Useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy. • LiveDye (green, Ex/Em=488/530nm), NucleiDye (red, Ex/Em= 535/617nm). |
| Usage notes | NucleiDye is a potential mutagen. Use appropriate precautions when handling this reagent. |
| Storage instructions | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Distinguishing between live and dead cells is very important for investigation of growth control and cell death. |
| Specifications | Kindly refer to the "Overview" tab. |
Author: Yu Sun, Han Yu, Hongzhou Peng, et al.
Publication Name: Journal of Materials Science & Technology
IF: 10.319
Magnesium (Mg) and its alloys have great potential as orthopedic implant candidates, which could release various bioactive substances during degradation. Degradation particles (DPs) are one of the degradation products, but the osteoimmunology effects are still unclear. In this study, the effect of DPs on the polarization of macrophages and their release of cytokines was investigated. The results verify that excessive generation of DPs from biodegradable Mg can induce macrophages to realize polarization of proinflammatory M1 phenotype. Moreover, macrophages secrete proinflammatory cytokines to inhibit the osteogenic differentiation of rat bone marrow stem cells (rBMSCs). This suggests that the effects of DPs should be considered when evaluating Mg-based implants. DPs continuously produced with the degradation of Mg-based implants may influence osseointegration.
Author: Sun Y, Chi X, Meng H, Ma M, Wang J, Feng Z, Quan Q, Liu G, Wang Y, Xie Y, Zheng Y, Peng J
Publication Name: Bioact Mater
IF: 8.724
Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits (NGCs). However, problems such as low loading efficiency and cell anoikis undermine the outcomes. Microcarriers are efficient 3D cell culture scaffolds, which can also prevent cell anoikis by providing substrate for adhesion during transplantation. Here, we demonstrate for the first time microcarrier-based cell transplantation in peripheral nerve repair. We first prepared macroporous chitosan microcarriers (CSMCs) by the emulsion-phase separation method, and then decorated the CSMCs with polylysine (pl-CSMCs) to improve cell affinity. We then loaded the pl-CSMCs with adipose-derived stem cells (ADSCs) and injected them into electrospun polycaprolactone/chitosan NGCs to repair rat sciatic nerve defects. The ADSCs-laden pl-CSMCs effectively improved nerve regeneration as demonstrated by evaluation of histology, motor function recovery, electrophysiology, and gastrocnemius recovery. With efficient cell transplantation, convenient operation, and the multiple merits of ADSCs, the ADSCs-laden pl-CSMCs hold good potential in peripheral nerve repair.