Overview
Opal™ 3-Plex Detection Kits provide a practical workflow for the simultaneous detection of up to 3 tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC is performed.
Catalog
Catalog # |
Product |
Size |
NEL810001KT |
Opal 3-Plex Manual Detection Kit (formerly Opal 4-Color Manual IHC Kit) |
Up to 50 Slides
|
NEL820001KT |
Opal 3-Plex Detection Kit (formerly Opal 4-Color Automation IHC Kit) |
Up to 50 Slides
|
Details
The Opal 3-Plex Manual Detection Kit contains:
- 3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
- DMSO
- 10X Spectral DAPI
- 1X Plus Manual Amplification Diluent
- 1X Antibody Diluent/Block
- 1X Opal Anti-Ms + Rb HRP
- AR6 Buffer
The Opal 3-Plex Detection Kit contains:
- 3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
- DMSO
- 10X Spectral DAPI
- 1X Plus Automation Amplification Diluent
- 1X Antibody Diluent/Block
- 1X Opal Anti-Ms + Rb HRP
Users should source primary antibodies and other workflow reagents separately. The Opal 3-Plex Detection Kits were optimized for use on the Mantra 2, Vectra 3, PhenoImager Fusion and PhenoImager HT Systems. Kits contain enough reagents to stain up to 50-slides at the recommended 1:100 or 1:150 dilution ratio.
Related products
PhenoImager™ HT