PANNORAMIC 250 Flash III - Digital Slide Scanner

Overview

The PANNORAMIC 250 Flash series digital scanners have been an ideal solution for digital pathology since their introduction in 2011. Thanks to its award-winning Flash scanning technology, PANNORAMIC 250 offers a versatile, high-quality, and high-speed scanning solution to high-volume scanning tasks.

3DHISTECH is continuously developing and improving the performance of its PANNORAMIC products to remain the fastest whole-slide scanner available on the market.

The PANNORAMIC 250 Flash III won the 2012 International Scanner Contest in the following categories: Scanning speed 20x; Scanning speed 40x and Image quality 40x.

The benefits of using PANNORAMIC Digital Slide Scanners

• Scan with the same device histology, cytology or molecular stained tissues with brightfield or fluorescence mode!
• High image quality even in case of thick samples: Thick, standard and thin sample compatible (3-6 µm)
• Scan daily routine and archive slides: Compatible with thick, standard and thin glass slides (0.8-1.2 mm)
• Saves scan time and storage space: Automatic tissue detection - no empty area scanning
• Suitable for suboptimal (fat, smear, blood etc.) samples: Flexible settings for various tissue properties
• Wide range of resolutions: Automatic 20x and 40x objective changer
• Compatibility with various coverslippers: Use film or glass coverslip
• LIS, HIS and immunostainer integration: Dual barcode compatible
• Flexible slide access: Direct scanning on slide server
• Quick zoom of images, true colour representation: Purpose built MRXS file format for lossless compression, supporting BF, FL, multilayer scanning
• Built-in quality control: Quantitative focus quality measurement

Key Features

Fast scanning and high throughput

The Flash III scanning technology offers fast scanning (35 sec/slide) and high throughput (60 slides/hour) (brightfield scanning, 41x optical magnification, 15mm x 15mm area, 15 FOVs Focus Distance, JPG compression, MRXS file format).

In order to comply with frequently used tender specifications, slide loading capacity has been increased to 300. This will reduce idle times and thus further increase slide throughput at both routine and research laboratories. 

Continuous loading

Continuous loading with vertical slide arrangement ensures easy access to slides in unloaded slide holder magazines. Also, a cover makes sure that no ambient light is shed upon your slides. The reworked slide loader allows for slides ranging from 0.9 mm to 1.2 mm of thickness.

There is no need to stop the scanning process in case of magazine changes and magazine unload, just keep on loading and unloading your slides into the system as they come!

Flash scanning technology

The light source offers very high stability in time over the lifetime with sub-millisecond switching times. Directly mounted on the scanner for maximum light coupling, the digital image is recorded with continuous slide movement by flashing the stroboscope light source in repetitive rhythm with perfect synchronization.

Minimized bleaching

The camera directly turns the light on for exposure time only.

Motorized objective and camera changer

Now you can have two high quality Zeiss Plan-Apochromat objectives in the scanner and also a camera for brightfield and one for fluorescence which you can change with a mouse click. The PANNORAMIC 250 Flash III can achieve up to 45x resolution in brightfield and up to 63x resolution in fluorescence.

A new level of fluorescence scanning

9 physical filter cubes and 45 logical fluorescence channels are available. A new camera and the new objective elevate fluorescence scanning to a new level.

New monochrome PCO edge 4.2 bi camera provides even better fluorescence image quality, especially for immunohistochemistry (IHC) applications and opal stainings. The sensitivity of the chip is extended in the 720-750 nanometer range, which reduces exposition time and further improves image quality. Also, the new camera works directly through a USB 3.0 port, no additional graphics card is necessary.

New optional 10x objective reduces fluorescence scanning time and provides further improved image quality.

Exceptional image quality

Exceptional image quality both in brightfield and up to 9-channel fluorescent scanning. Up to 80x brightfield and 60x fluorescence magnification by default, all available within the same instrument, coupled with an advanced FISH scanning technique.

New slide sensor

The new slide sensor ensures that empty slots are automatically skipped during scanning, further accelerating the scanning process.

Application

The PANNORAMIC 250 Flash III is a great choice for large laboratories looking for high-throughput and high-quality scanning solution in brightfield and fluorescence.

Scanner Software for Research

PANNORAMIC Scanners are powered by the PANNORAMIC Scanner Software for Research, which provides versatile, fast and high-quality slide scanning according to the imaging needs of cancer research laboratories, pharmaceutical companies or medical research institutes. Its key features include

• New Graphical User Interface (GUI)
• Scanning profiles for individual slides, multiple scanning locations and separate scanning of TMA slides for flexibility
• Several focus modes, new intensity stitching algorithm, two multilayer scanning options and further improved deconvolution for superior image quality
• Highly adjustable software and hardware settings
• Customizable user interface and scanning workflow
• Built-in slide conversion into the following file formats: DICOM, Tiled TIFF

Complementary Software Solutions

The 3DHISTECH software product portfolio is the most comprehensive one on the market. Whether it is tissue microarrays, IHC stainings, quantitative measurements, e-learning application and more, 3DHISTECH offers solutions for your research work.