This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.
• Fast: 15 minutes
• Simple: No restriction enzyme digestions.
• High efficiency: > 95% cloning efficiency.
• Seamless: No extra sequences introduced.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C