pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
• 5 minutes fast ligation of Pfu-amplified PCR products.
• Ampicillin resistance.
• T7 promoter primer and T7 terminator primer for sequencing.
• Bacteriophage T7lac promoter for high level expression.
• N-terminal 6×His tag for easy purification.
• Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
• E1 Expression Plasmid included as negative control.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; Others at -20°C for nine months.
Shipping
Dry ice (-70°C).
Product Contents
Component |
CE111-01 (10 rxns) |
pEASY®-Blunt E1 Expression Vector (15 ng/μl) |
10 μl |
E1 Expression Plasmid (Negative Control) (15 ng/μl) |
10 μl |
EControl Template (5 ng/μl) |
10 μl |
EControl Forward Primer (10 μM) |
10 μl |
EControl Reverse Primer (10 μM) |
10 μl |
T7 Promoter Primer (10 μM) |
50 μl |
T7 Terminator Primer (10 μM) |
50 μl |
Trans1-T1 Phage Resistant Chemically Competent Cell |
5×100 μl |