pEASY®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.
•5 minutes fast ligation of Taq-amplified PCR products.
•Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter and SP6 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for nine months