This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-7 fragments.
• Fast: 15 minutes
• Simple: No restriction enzyme digestions.
• High efficiency: > 95% cloning efficiency.
• Seamless: No extra sequences introduced.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cells at -70°C for six months; others at -20°C for one year.
Shipping
Dry ice (-70°C )
Product Contents
Component |
CU101-01 |
CU101-02 |
CU101-03 |
2×Assembly Mix |
50 μl |
100 μl |
3×100 μl |
Linearized pUC19 Control Vector (10 ng/μl) |
3 μl |
6 μl |
18 μl |
Control Insert (1 kb, 20 ng/μl) |
3 μl |
6 μl |
18 μl |
Trans1-T1 Phage Resistant Chemically Competent Cell |
5×100 μl |
10×100 μl |
30×100 μl |