PhenoCycler Alexa Fluor™ 647 Reporters

Overview

PhenoCycler Reporters are included with each PhenoCycler Antibody and PhenoCycler Barcode Kit or can be ordered individually. For each PhenoCycler imaging cycles, three PhenoCycler Reporters, each with a spectrally distinct dye, are applied to the stained tissue to assay the corresponding antibody barcode. The tissue is imaged, revealing the fluorescence signal levels of the first three targets plus a nuclear stain.

Each PhenoCycler antibody is conjugated to a unique oligonucleotide sequence, referred to as the PhenoCycler Barcode. Each PhenoCycler Barcode, in turn, is complementary to a unique PhenoCycler Reporter. Reporter is comprised of a fluorescent dye and a short oligonucleotide.

Therefore, by ordering the reporters below from the list below, you will still need to order your own antibody and barcodes to complement with the PhenoCycler Barcode containing the antibody with the conjugated barcode.

Please refer HERE if you would like to purchase the inventoried antibodies containing the conjugated barcode and their complementary fluorophore.

PhenoCycler Component Reference Map

Please note that you will still need to purchase the following items:

• Antibodies
  a) List of Screened and Community Based Antibodies
  b) ELK Biotech Antibodies
• Barcode
• PhenoCycler System Kits & Reagents

Catalog

Related products

PhenoCycler™ - Fusion System