Overview

The list containing antibodies that will work with PhenoCycler have been “Screened” and tested in the Akoya Biosciences lab internally or tested and worked by the “community”. This is as a reference to any uses who would like to capitalise on PhenoCycler’s Chemistry and would like to integrate their own antibodies to this workflow.

Please refer HERE if you would like to purchase the inventoried antibodies containing the conjugated barcode and their complementary fluorophore.

PhenoCycler Component Reference Map

Please note that you will need to purchase the following items:

Details

PhenoCycler Screened and Community Based Antibodies
Target	Phenotype	Tissue	Tissue Type	Category
CD47 (TSP-1)	"Don't eat me"	Human FFPE	Tonsil, Prostate	Screened
CD227 (Muc-1)	epithelial cells	Human FFPE	Various, Meningioma	Screened
Helios	Transcription Factor	Human FFPE	Tonsil, Prostate	Screened
CXCR2	Myeloid (Neutrophil)	Human FFPE	Tonsil, Prostate	Screened
AMACR	Tumor	Human FFPE	Tonsil, Prostate	Screened
PSMA	Tumor	Human FFPE	Tonsil, Prostate	Screened
CD86	Myeloid	Human FFPE	Tonsil, Prostate	Screened
CD74	Myeloid	Human FFPE	Tonsil, Prostate	Screened
CD172	Myeloid	Human FFPE	Tonsil, Prostate	Screened
Vimentin	Mesenchymal cells	Mouse FFPE	Brain, Spleen, Tumor	Screened
F4/80	macrophages	Mouse FFPE	Spleen, Tumor	Screened
TIM3	T cells supression, macrophages, denditric cells	Human FFPE	Various	Screened
CD206	M2 Macrophages	Human FFPE	Tonsil, Prostate, Tumor TMA	Screened
CD1c	Myeloid (Dendritic)	Human FFPE	Tonsil, Prostate	Screened
LOX-1	Endothelial, Macrophages, Cardiomyocytes	Human FFPE	Tonsil, Prostate	Screened
NKp46	NK cell	Human FFPE	Tonsil, Bladder	Screened
NKG2A	NK cell	Human FFPE	Tonsil, Bladder	Screened
DNAM-1	NK cell	Human FFPE	Tonsil, Bladder	Screened
CXCR3	NK cell	Human FFPE	Tonsil, Bladder	Screened
CD56	NK cell	Human FFPE, Mouse FFPE	Tonsil, Spleen, Tumor	Screened
NKG2D	NK cell	Human FFPE	H&N, Ovarian Cancer	Screened
CD16	NK cell	Human FFPE	Tonsil, Prostate	Screened
CD16	NK cell	Human FFPE	H&N, Ovarian Cancer	Screened
CD56	NK cell	Human FFPE	H&N, Ovarian Cancer	Screened
CD163	Myeloid	Human FFPE	Various	Screened
Keratin5	Basal keratinocytes/Basal tumor subtype	Human FFPE	Breast	Screened
Keratin8	Luminal epithelial cells/Luminal tumor subtype	Human FFPE	Breast	Screened
Keratin19	Luminal epithelial cells/Luminal tumor subtype	Human FFPE	Breast	Screened
Col1A1	ECM organization	Mouse FFPE	Spleen, Tumor, Brain	Screened
camKII	Excitatory neurons	Rat FF	Brain	Screened
CCR2	Monocytes/macrophages	NHP FFPE	lung	Screened
CD206	Macrophages	NHP FFPE	lung	Screened
HER2	Luminal breast cancer (Huang et al 2021)	Human FFPE	Breast	Screened
CD28	T cells	NHP FFPE	lung	Screened
CNP1	Dendritic cells, Langerhans cells	Rat FF	Brain	Screened
ERAP1	Neurons	Human FFPE	Ovarian	Screened
CD11b	Myeloid cells	Human FFPE	Various	Screened
MICA/B	"Kill me"	Human FFPE	H&N, Ovarian, TMA	Screened
P21	Negative regulator of cell cycle/cell cycle arrest	Human FF	Brain Organoids	Screened
P62	Autophagy marker, defective protein clearance	Human FF	Brain Organoids	Screened
STING	Microglia, vasculature, immune cells, tumor cells, stimulator of interferon genes (Anti-tumor)	Human FFPE	Ovarian	Screened
TfR	Endothelial cells	Human FFPE, NHP FFPE	Brain	Screened
MAP-2	Neurons	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
H2A.X	DNA damage	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
GFAP	Astrocytes	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
PSD-95	Synaptic marker/neurons	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
NeuN	Neurons	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
Synaptophysin	Synapses	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
Olig-2	Oligodendrocyte	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
VGLUT1	Neurons	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
Doublecortin	Neurons	Human FFPE, Mouse FFPE	Brain	Screened
MBP	Oligodendrocyte	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
GAD67	Neurons	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
Serotonin Tr	Neurons	Human FFPE, Mouse FFPE	Brain	Screened
cTIP	Neurons	Human FFPE, Mouse FFPE	Brain	Screened
Calbindin	Neurons	Human FFPE, Mouse FFPE	Brain	Screened
LamB1	Nucleated cells	Human FFPE, Mouse FFPE	Brain	Screened
Neurofilament	Neurons	Human FFPE, Mouse FFPE, NHP FFPE	Brain	Screened
TMEM119	Microglia	Human FFPE, Mouse FFPE	Brain	Screened
AQP4	Astrocytes	Human FFPE, Mouse FFPE	Brain	Screened
A-beta	Neurons, glia	Human FFPE, Mouse FFPE	Brain	Screened
Iba1	Macrophages/ Microglia	Human FF	Pancreas	Community
CD31	Endothelial cells	Mouse FFPE	Brain	Screened
P-Tau (S396)	Neurons	Mouse FFPE	Brain	Screened
CD68	Macrophages, monocytes	Mouse FFPE	Brain	Screened
APOE	Hepatocytes, macrophages, and neurons	Human FFPE	Brain	Screened
Tau (pSer202)	Neurons	Human FFPE	Brain	Screened
Tau	Neurons	Human FFPE	Brain	Screened
Claudin-5	Vascular	Human FFPE, NHP FFPE	Brain	Screened
ChAt	Neurons	Human FFPE, NHP FFPE	Brain	Screened
TH	Neurons	Human FFPE, NHP FFPE	Brain	Screened
Sox9	Chondrocytes, Sertoli Cells, Stem and Progenitor Cells, Neural Cells	Human FFPE, NHP FFPE	Brain	Screened
GAD1	Neurons	Human FFPE, NHP FFPE	Brain	Screened
VIAAT	Neurons, GABAergic and glycinergic cells	Human FFPE, NHP FFPE	Brain	Screened
LRP1	Neurons, hepatocytes, and immune cells	Human FFPE, NHP FFPE	Brain	Screened
SOD1	Neurons, erythrocytes, and immune cells	Human FFPE, NHP FFPE	Brain	Screened
IGF-IR	Fibroblasts, muscle cells, and immune cells	Human FFPE, NHP FFPE	Brain	Screened
NPR-C?	Smooth muscle cells, fibroblasts	NHP FFPE	Brain	Screened
LRP2?	Kidney cells and Epithelial cells	NHP FFPE	Brain	Screened
PTH1R?	Bone cells, kidney cells	NHP FFPE	Brain	Screened
SGLT2?	Renal proximal tubule cells	NHP FFPE	Brain	Screened
ASCT2	Neurons, tumor cells and immune cells	Human FFPE	Various	Screened
IDH2	Tumor cells	Human FFPE	Various	Screened
MC Tryptase	Mast cells	Human FFPE	Various	Screened
LC3B	Tumor cells	Human FFPE	Various	Screened
Cleaved PARP	Apoptotic cells	Human FFPE	Various	Screened
BCL-XL	Lymphocytes and tumor cells	Human FFPE	Various	Screened
NaKATPase	Neurons, muscle cells and epithelial cells	Human FFPE	Various	Screened
Cytochrome c	Apoptotic cells	Human FFPE	Various	Screened
CD227	Epithelial cells	Human FFPE	Various	Screened
LEF1	T cells	Human FFPE	Various	Screened
C1Qa	Kupffer cells (macrophages), monocytes	Human FFPE	Various	Screened
GLUT1	Erythrocytes and tumor cells	Human FFPE	Various	Screened
Cyclin D1	Proliferating cells	Human FFPE	Various	Screened
BAD	Apoptotic cells	Human FFPE	Various	Screened
BAX	Apoptotic cells	Human FFPE	Various	Screened
CD138	Plasma cells	Human FFPE	Various	Screened
pNRF2	Cells undergoing oxidative stress	Human FFPE	Various	Screened
CPT1A	Cells involved in fatty acid metabolism	Human FFPE	Various	Screened
CD2	T cells and NK cells	Human FFPE	Various	Screened
CD1a	Dendritic cells	Human FFPE	Various	Screened
ATPA5	Metabolic: TCA cycle	Human FFPE	Various	Screened
Citrate Synthase	Metabolic: TCA cycle	Human FFPE	Various	Screened
CK17	Epithelial cells	Human FFPE	Various	Screened
CD7	T cells and NK cells	Human FFPE	Various	Screened
G6PD	Metabolic: Pentose Phosphate Pathway	Human FFPE	Various	Screened
CD69	T cells and NK cells	Human FFPE	Various	Screened
HLA-DPB1	B cells, dendritic cells and macrophages	Human FFPE	Various	Screened
Pax5	B cells	Human FFPE	Various	Screened
CCR6	B cells	Human FFPE	Various	Screened
AXL	Signaling: Immune evasion	Human FFPE	Various	Screened
CD15	Granulocytes	Human FFPE	Various	Screened
CK19	Epithelial cells	Human FFPE	Various	Screened
CD163	M2 Macrophages	Human FFPE	Various	Screened
SDHA	Metabolic: TCA cycle	Human FFPE	Various	Screened
LaminB1	Ubiquitous: Nuclear lamen	Human FFPE	Various	Screened
ZEB1	Tumor cells: epithelial-mesenchymal transition	Human FFPE	Various	Screened
HK1	Metabolic: Glycolysis	Human FFPE	Various	Screened
LDHA	Metabolic: Glycolysis	Human FFPE	Various	Screened
MMP-9	Metabolic: Invasion	Human FFPE	Various	Screened
pRPS6	Signaling: Proliferation	Human FFPE	Various	Screened
Galectin 9	Signaling: Apoptosis	Human FFPE	Various	Screened
CD57	NK cells and cytotoxic T cells	Human FFPE	Various	Screened
Beclin-1	Stress: Autophagy	Human FFPE	Various	Screened
ZAP70	T cells and NK cells	Human FFPE	Various	Screened
NOX2	Phagocytes	Human FFPE	GBM	Screened
CD172 (SIRPα)	Myeloid cells, stem cells, neurons	Human FFPE	GBM	Screened
CD161	NK cells and some lymphocytes	Human FFPE	lymph node; nasopharyngeal carcinoma	Screened
p21	Senescence	Human FFPE	nasopharyngeal carcinoma	Screened
Glucagon	pancreatic alpha cells	Mouse FFPE Xenograft, Human FFPE	Kidney	Screened
Insulin	pancreatic beta cells	Mouse FFPE Xenograft, Human FFPE	Kidney	Screened
Cardiac Troponin	Myocardial injury (cardiomyyocytes)	Human FFPE	Heart	Screened
Integrin beta 1 (CD29)	Leukocytes, adipose-derived stem cells and BM-MSCs	Human FFPE	Kidney	Screened
PR	PR positive tumor cells	Human FFPE	Breast	Screened
FAP	Fibroblasts	Human FFPE	Breast, TMA	Screened
Transgelin	smooth muscle cells	Human FFPE	TMA Tumor	Screened
Ficolin	Monocytes,neutrophils	Human FFPE	TMA Tumor	Screened
CD54	Endothelial cells, activated lymphocytes	Human FFPE	TMA Tumor	Screened
CD27	B and T cell activation	Human FFPE	TMA Tumor	Screened
CD20	B cells	Mouse FFPE	Spleen, Tumor	Screened
CD8	Cytotoxic T cells	Mouse FFPE	Spleen, Tumor	Screened
CD11c	Dendritic cells	Mouse FFPE	Spleen, Tumor	Screened
CD31	endothelal cells	Mouse FFPE	Spleen, Tumor	Screened
CD44	Cancer Stem Cells	Mouse FFPE	Spleen, Tumor	Screened
Foxp3	T cells (T reg)	Mouse FFPE	Spleen, Tumor	Screened
CD3e	Mature T cells	Mouse FFPE	Spleen, Tumor	Screened
CD36	Human Adipocyte Progenitors	Mouse FFPE	Spleen, Tumor	Screened
CD98hc (SLC3A2)	Endothelial cells, neuropil	Human FFPE, NHP FFPE	Brain	Screened
CD206	Macrophages (M2)	Mouse FFPE	Spleen, Tumor	Screened
FcRγ	Myeloid cells, Nk cells, Macrophages	Mouse FFPE	Spleen, Tumor	Screened
CGRP	sensory nerves; also expressed by fibroblasts, keratinocytes,T lymphocytes, B lymphocytes, and monocytes	Mouse FF	Bone Marrow	Screened
LePR	perivascular cells	Mouse FF	Bone Marrow	Screened
Iba1	Glia	Mouse FFPE	Spleen, Tumor	Screened
Flk2	hematopoietic stem cell differentiation	Mouse FF	Bone Marrow	Screened
CD68	Macrophages, monocytes	Mouse FFPE	Spleen, Tumor	Screened
CD45	nucleated Hematopoietic cells (Pan Immune)	Mouse FFPE	Spleen, Tumor	Screened
S100A9	Neutrophils, macrophages	Mouse FFPE	Spleen, Tumor	Screened
Ly6g	Peripheral neutrophils, monocytes, granulocytes	Mouse FFPE	Spleen, Tumor	Screened
MPO	neutrophils	NHP FFPE	Spleen, Retina, Lung	Screened
Calprotectin	neutrophils	NHP FFPE	Spleen, Retina, Lung	Screened
CD3e	T cells	NHP FFPE	Spleen, Retina, Lung	Screened
CD8a	cytotoxic T cells	NHP FFPE	Spleen, Retina, Lung	Screened
SLAM (CD150)	peripheral blood hematopoietic stem and progenitor cells	Mouse FF	Bone Marrow	Screened
Nkp46	NK cells	Mouse FF	Bone Marrow	Screened
Fcgr(CD32/CD16)	NK cells	Mouse FF	Bone Marrow	Screened
Perlipin	adipocytes	Mouse FF	Bone Marrow	Screened
F4/80	macrophages	Mouse FF	Bone Marrow	Screened
CD41	platelets and megakaryocytes	Mouse FF	Bone Marrow	Screened
CD115	monocytes, macrophages	Mouse FF	Bone Marrow	Screened
Endomucin	endothelium	Mouse FF	Bone Marrow	Screened
Tyrosine Hydroxylase	dopaminergic neurons	Mouse FF	Bone Marrow	Screened
CD105	angiogenic endothelial cells	Mouse FF	Bone Marrow	Screened
CD19	B cells	Human FFPE	Various	Screened
CD48	lymphocytes, monocytes	Mouse FF	Bone Marrow	Screened
CD15	myeloid cells	Human FFPE	Various	Screened
CD16	NK cells	Human FFPE	Various	Screened
B7H3/CD276	Fibroblasts	Human FFPE	Human FFPE Head and Neck, Ovarian, Multitumor Cancer TMA	Screened
CD227	epithelial cells	Human FFPE	Breast Organoids	Screened
IRF4/MUM1	B cells, plasma cells	Human FFPE	Tonsil, Multi-tissue TMA	Screened
CD278	activated CD4+ and CD8+ T cells	Human FFPE	Tonsil	Screened
CD30	activated T cells, NK cells, B cells	Human FFPE	Tonsil	Screened
CD5	T cells	Human FFPE	Tonsil	Screened
ER	nuclear and membrane receptor localized in a subset mature luminal cells and luminal progenitor cells (Rosenbluth et al 2020)	Human FFPE	Breast Organoids	Screened
GATA3	Transcription factor expressed in the luminal A subtype (Voduc et al 2008)	Human FFPE	Breast Organoids	Screened
p53	Tumor supressor and transcription factor, expressed at low levels in unstressed cells (Aubrey et al 2017; Zhang et al 2018)	Human FFPE	GBM	Screened
CD25 (IL2RA)	T cells	Human FFPE	Various	Screened
LMP1	Memory B cells in tonsil following Epstein-Barr virus infection (Babcock and Lawson 2000). Germinal center centroblasts and centrocytes (Babcock et al 2000)	Human FFPE	Tonsil	Screened
EBNA1	Memory B cells in tonsil following Epstein-Barr virus infection (Babcock and Lawson 2000). Germinal center centroblasts and centrocytes (Babcock et al 2000)	Human FFPE	Tonsil	Screened
EBNA2	Naive B cells (Babcock et al 2000)	Human FFPE	Tonsil	Screened
LMP2A	EBV infected B cells (Rovedo and Longnecker 2007)	Human FFPE	Tonsil	Screened
CD30	B lymphoblasts (Steiniger et al 2020)	Human FFPE	Tonsil	Screened
EBNA2	Naive B cells (Babcock et al 2000)	Human FFPE	Tonsil	Screened
LMP1	Memory B cells in tonsil following Epstein-Barr virus infection (Babcock and Lawson 2000). Germinal center centroblasts and centrocytes (Babcock et al 2000)	Human FFPE	Tonsil	Screened
EBVEaD	EBV infected B cells (Thermofisher)	Human FFPE	Tonsil	Screened
S100A4	Fibroblasts and immune cells	Human FFPE	Various	Screened
Tim-3	CD4 helper T cells and CD8 T lymphocytes (Wolf et al 2019)	Human FFPE	Tonsil	Screened
NFkB p65	Infllammatory marker, Activated B cells	Human FFPE	Tonsil	Screened
ERG	pan endothelial marker, lymphovascular invasion (Kim et al 2013)	Human FFPE	Breast	Screened
Active YAP	Epigenetics	Human FFPE	Tonsil	Screened
BID	Epigenetics	Human FFPE	Tonsil	Screened
H3Me3K9	Epigenetics	Human FFPE	Tonsil	Screened
H3Me3K27	Epigenetics	Human FFPE	Tonsil	Screened
H4MeK20	Epigenetics	Human FFPE	Tonsil	Screened
H3Me3K36	Epigenetics	Human FFPE	Tonsil	Screened
H3AcK9	Epigenetics	Human FFPE	Tonsil	Screened
H3Me123K79	Epigenetics	Human FFPE	Tonsil	Screened
HDAC2	Epigenetics	Human FFPE	Tonsil	Screened
Dnmt1	Epigenetics	Human FFPE	Tonsil	Screened
HP1 alpha	Epigenetics	Human FFPE	Tonsil	Screened
Brd4	Epigenetics	Human FFPE	Tonsil	Screened
pS139 gamma histone H2AX	Epigenetics, double stranded DNA breaks	Human FFPE	TMA Tumor	Screened
pY1068/Y1173 EGFR	EGFR signaling	Human FFPE	TMA Tumor	Screened
CA9	pH regulation/ Hypoxia	Human FFPE	TMA Tumor	Screened
pT202 ERK1 + Y204 ERK2	ERK1/ ERK2 signaling	Human FFPE	TMA Tumor	Screened
TGF beta	TGF beta signaling	Human FFPE	TMA Tumor	Screened
pT202 ERK1 + T185 ERK2	ERK1/ ERK2 signaling	Human FFPE	TMA Tumor	Screened
CD27	Lymphocyte co-stimulation	Mouse FF	Bone Marrow	Community
CD34	hematopoetic stem cells, hematopoetic progenitors, vascular endothelial cells	Mouse FF	Bone Marrow	Community
CD47	Phagocytosis regulation, "don't eat me"	Mouse FF	Bone Marrow	Community
CD62L	T cell memory	Mouse FF	Bone Marrow	Community
CD117	hematopoetic stem cells, hematopoetic progenitors	Mouse FF	Bone Marrow	Community
CTLA4	Negative T cell costimulation	Mouse FF	Bone Marrow	Community
CD144/VE-Cadherin	Endothelial cells	Mouse FF	Bone Marrow	Community
CD206	M2 Macrophage	Mouse FF	Bone Marrow	Community
TCR Gamma/Delta	Gamma Delta T cells, peptide and lipid antigen recognition	Mouse FF	Bone Marrow	Community
Arginase 1	MDSCs/ Alternative activated Macrophages	Human FF	Pancreas	Community
C-peptide	Beta cells of pancreas, Insulin secretion	Human FF	Pancreas	Community
CD36	Atheroslcerosis	Human FF	Pancreas	Community
CD86	Lymphocyte acitvation/ immune checkpoint marker	Human FF	Pancreas	Community
CD163	M2 macrophage	Human FF	Pancreas	Community
CD206	M2 Macrophage	Human FF	Pancreas	Community
Chromagranin A	Neuroendocrine cells	Human FF	Pancreas	Community
Ghrelin	Endocrine cells of GI tract	Human FF	Pancreas	Community
Glucagon	alpha cells of pancreatic islets of Langerhans	Human FF	Pancreas	Community
Pancreatic Polypeptide/PP	PP cells of Pancreas	Human FF	Pancreas	Community
Somatostatin	Delta cells of Pancreas	Human FF	Pancreas	Community
CD10	B lymphoblast cell development and leukemia	Human FFPE	Tonsil	Community
CD123	Interleukin-3 receptor, plasmacytoid dendritic cells, various types of lukemia	Human FFPE	Tonsil	Community
CD123	Interleukin-3 receptor, plasmacytoid dendritic cells, various types of lukemia	Human FFPE	Tonsil	Community
CLEC9A	Dendritic cell subsets	Human FFPE	Tonsil	Community
DR5	TRAIL activated cell death signaling receptor	Human FFPE	Tonsil	Community
NSE	Neurons and peripheral neuroendocrine cells	Human FFPE	Tonsil	Community
CD93	Plasma cell/B cell	Human FFE	Meningioma	Screened
IGFBP7	Insulin like growth factor binding protein	Human FFE	Meningioma	Screened
PLA2G10	Phospholipase A2	Human FFE	Meningioma	Screened
CTLA4	Activated T cell	Human FFPE	Various	Screened
SOX10	Transcription factor/Marker for malignant melanoma	Human FFE	Melanoma	Screened
p21	Negative regulator of cell cycle/cell cycle arrest	Human FFE	Breast	Screened
PTEN	Tumor supressor	Human FFE	Breast, Melanoma	Screened
p120	Predictor of outcome in lobular neoplasia	Human FFE	Breast	Screened
Bcl6	Transcription factor for regulator of TFH cells	Human FFE	Tonsil	Screened