RmesFect ™ Transfection Reagent

Overview

RmesFect™ Transfection Reagent is based on the TEE-technology specifically designed for mRNA transfection with high efficiency and low toxicity. mRNA transfection provides several advantages over plasmid DNA delivery :

• No need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm.
• Faster protein expression than DNA transfection
• No genomic integration
• Protein expression in a total promoter-independent manner

RmesFect™ is specifically designed for in vitro mRNA (dsRNA) transfection in a large variety of cells. RmesFect™ transfection reagent principal advantages:

• Highly efficient : achieve mRNA delivery in all cells
• Ready-to-use: no need for additional buffer
• Low nucleic acid amount : minimized toxicity
• Protects mRNA against degradation
• Compatible with any culture medium
• Serum compatible : Medium changed not required

• 500 µL: 125-250 transfections with 1µg of mRNA
• 1 mL: 250-500 transfections with 1µg of mRNA
• 5 mL: 1250-2500 transfections with 1µg of mRNA

Shipping Conditions: Room temperature

 Applications

• Perfect for all your mRNA transfection applications:

  • mRNA vaccines

  • primary cells transfection

  • regenerative medicine

  • cell reprogramming

  • iPs generation

RECOMMENDED FOR: Transfection of mRNA in primary cells and cell lines.

Results

Figure 1: Several cell lines were transfected with 0.5 μg of mRNA encoding GFP protein and RmesFect™ transfection reagent (ratio 2:1 for RAW264.7,3:1 for COS7 and CHO-K1 and 4:1 for HeLa cells). After 20 min of incubation at room temperature, complexes were added onto the cells in a dropwise manner. 24 H after, transfection efficiency was measured by FACS analysis.

Figure 2: Several cell lines were transfected with  mRNA encoding GFP protein and RmesFect™ transfection reagent. 24 H after, transfection efficiency was measured by fluorescence microscopy.