Synthetic Minimal Media (SD media) are based upon Yeast Nitrogen Base without Amino Acids and supplemented with a carbon source like glucose, galactose, raffinose, succinate or a combination of carbon sources.
Synthetic Minimal Media (SD media) are based upon Yeast Nitrogen Base without Amino Acids and supplemented with a carbon source like glucose, galactose, raffinose, succinate or a combination of carbon sources. The Synthetic Minimal Media are available with or without Agar premixed.
Technical Data
SD Agar / Glucose
SD Broth / Glucose
SD Agar / Glucose w/o Phosphate
SD Broth / Glucose w/o Phosphate
SD Agar / Galactose
SD Broth / Galactose
SD Agar / Raffinose
SD Broth / Raffinose
SD Agar / Succinate
SD Broth / Succinate
SD Agar / Galactose and Raffinose
SD Broth / Galactose and Raffinose
Nitrogen Source g/l
Ammonium Sulphate
5
5
5
5
5
5
5
5
5
5
5
5
Carbon Source g/l
Glucose.H2O
20
20
20
20
–
–
–
–
–
–
–
–
Galactose
–
–
–
–
20
20
–
–
–
–
20
20
Raffinose
–
–
–
–
–
–
20
20
–
–
10
10
Succinate
–
–
–
–
–
–
–
–
33
33
–
–
Vitamins µg/l
Biotin
2
2
2
2
2
2
2
2
2
2
2
2
Ca-Panthotenate
400
400
400
400
400
400
400
400
400
400
400
400
Folic Acid
2
2
2
2
2
2
2
2
2
2
2
2
Inositol
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
2000
Nicotinic Acid
400
400
400
400
400
400
400
400
400
400
400
400
p-Aminobenzoic Acid
200
200
200
200
200
200
200
200
200
200
200
200
Pyridoxine HCl
400
400
400
400
400
400
400
400
400
400
400
400
Riboflavin
200
200
200
200
200
200
200
200
200
200
200
200
Thiamine HCl
400
400
400
400
400
400
400
400
400
400
400
400
Trace Elements µg/l
Boric Acid
500
500
500
500
500
500
500
500
500
500
500
500
Copper Sulfate
40
40
40
40
40
40
40
40
40
40
40
40
Potsssium Iodide
100
100
100
100
100
100
100
100
100
100
100
100
Ferric Chloride
200
200
200
200
200
200
200
200
200
200
200
200
Manganese Sulfate
400
400
400
400
400
400
400
400
400
400
400
400
Sodium Molybdate
200
200
200
200
200
200
200
200
200
200
200
200
Zinc Sulfate
400
400
400
400
400
400
400
400
400
400
400
400
Minerals g/l
KH2PO4
1
1
–
–
1
1
1
1
1
1
1
1
Magnesium Sulphate.anh
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
Sodium Chloride
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Calcium Chloride.anh
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Agar g/l
Agar
18
–
18
–
18
–
18
–
18
–
18
–
Product Range
SD Agar / 2% Glucose
SD Broth / 2% Glucose
SD Agar / 2% Glucose w/o Phosphate (KH2PO4)
SD Broth / 2% Glucose w/o Phosphate (KH2PO4)
SD Agar / 2% Galactose
SD Broth / 2% Galactose
SD Agar / 2% Raffinose
SD Broth / 2% Raffinose
SD Agar / 3.3% Succinate
SD Broth / 3.3% Succinate
SD Agar / 2% Galactose / 1% Raffinose
SD Broth / 2% Galactose / 1% Raffinose
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