Renilla luciferase catalyzes the oxidation of coelenterazine to form coelenteramide, and produces bioluminescence in the process. TransDetect® Single-Luciferase (Renilla) Reporter Assay Kit uses coelenterazine as a substrate to detect the activity of Renilla luciferase reporter gene. It has the characteristics of rapid detection, high sensitivity, wide detection range, and no interference of endogenous activity of cells.
Storage
The kit stored at -20°C for one year. Cell Lysis Buffer can be stored at -20°C for one year. Prepared Luciferase Reaction Reagent II should be stored in aliquots in dark at -20°C for one month or at -70°C for one year.