TransScript® Green Two-Step qRT-PCR SuperMix is a two-step fluorescent quantitative RT-PCR (qRT-PCR) system providing highly efficient synthesis and amplification. 5×TransScript® All-in-One SuperMix for qPCR together with gDNA Remover allows simultaneous first-strand cDNA synthesis and gDNA removal. 5×TransScript® All-in-One No-RT Control SuperMix for qPCR is supplied as a control test in absence of reverse transcriptase, which is used to judge whether the qPCR template comes from cDNA or not. PerfectStartTM Green qPCR SuperMix is included as a component for qPCR amplification.
Highlights
• Simultaneous genomic DNA removal and cDNA synthesis to minimize contamination.
• High amplification efficiency, specificity and sensitivity, ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
•Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).
Applications
Multiple copy and low copy gene detection.
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70°C)
Product Contents
Component |
AQ201-01 |
5×TransScript® All-in-One SuperMix for qPCR |
200 μl |
5×TransScript® All-in-One No-RT Control SuperMix for qPCR |
20 μl |
gDNA Remover |
50 μl |
2×PerfectStartTM Green qPCR SuperMix |
3×1 ml |
Passive Reference Dye (50×) |
120 μl |
RNase-free Water |
1 ml |