TransScript® II Green One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers to achieve one step from reverse transcription to qPCR in a single tube.
Highlights
• TransScript® II Green One-Step RT/RI Enzyme Mix is used to efficiently synthesize first-strand cDNA from RNA and 2×PerfectStartTM Green One-Step qPCR SuperMix is used for qPCR, enabling simple procedures to minimize the chance of contamination.
• High sensitivity and high specificity to ensure accurate results.
Applications
• High-copy and low-copy gene detection
• Detection of RNA virus or trace amounts of RNA
Storage
at -20°C away from light for two years
Shipping
Dry ice (-70°C)
Product Contents
Component |
AQ311-01 |
AQ311-02 |
TransScript® II Green One-Step RT/ RI Enzyme Mix |
40 μl |
160 μl |
PerfectStart® Green One-Step qPCR SuperMix (2×) |
1 ml |
4×1 ml |
Passive Reference Dye (50×) |
40 μl |
160 μl |
RNase-free Water |
1 ml |
4×1 ml |