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Bio Reagents & Consumables
qPCR and qRT-PCR SuperMixes
TransScript® II Green Two-Step qRT-PCR SuperMix
TransScript® II Green Two-Step qRT-PCR SuperMix is a two-step fluorescent quantitative RT-PCR (qRT-PCR) system providing highly efficient synthesis and amplification. 5×TransScript® II All-in-One SuperMix for qPCR together with gDNA remover allows simultaneous first-strand cDNA synthesis and gDNA removal at 42°C -55°C . 5×TransScript® II All-in-One No-RT Control SuperMix for qPCR is supplied as a control test in absence of reverse transcriptase, which is used to judge whether the qPCR template comes from cDNA or not. PerfectStartTM Green qPCR SuperMix is included as a component for qPCR amplification.
Highlights
• Simultaneous genomic DNA removal and cDNA synthesis to minimize contamination.
• High amplification efficiency, specificity and sensitivity, ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).
Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70°C)
Product Contents
| Component | AQ301-01 |
| 5×TransScript® All-in-One SuperMix for qPCR | 200 μl |
| 5×TransScript® All-in-One No-RT Control SuperMix for qPCR | 20 μl |
| gDNA Remover | 50 μl |
| 2×PerfectStartTM Green qPCR SuperMix | 3×1 ml |
| Passive Reference Dye (50×) | 120 μl |
| RNase-free Water | 1 ml |
| Product Contents | Kindly refer to the "Overview" tab. |
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