TransScript® II Two-Step RT-PCR SuperMix is a two-step RT-PCR kit with high synthesis capacity and high amplification efficiency. The first-strand cDNA is synthesized efficiently from RNA by reverse transcription with 5×TransScript® II All-in-One SuperMix for PCR at 42°C-55°C; and amplified by PCR with 2×TransTaq® HiFi PCR SuperMix II. This kit contains all the reagents for reactions from RNA to cDNA and PCR amplification for this cDNA.