TransScript® Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)

The kit provides all the necessary reagents for reverse transcription reaction (including TransScript® Uni RT, RNase Inhibitor, Anchored Oligo (dT)₂₀ Primer, Random Primer (N9), dNTPs, Buffer). It is provided at 5× concentration and used by only adding gDNA remover, RNA template and H₂O at 42°C-65°C for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×TransScript® Uni All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR.

Highlights

• Board range reaction temperature (42°C-65°C).

• "All-in-One SuperMix" form: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H₂O

• High synthesis efficiency enabled by optimal ratio of Oligo(dT)₂₀ Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically high synthesis efficiency for short cDNA.

• Only 5 minutes for reverse transcription.

• High compatibility with qPCR reagents.

Applications

High-copy number and low-copy number gene detection

Storage
at -20°C for two years

Shipping
Dry ice (-70°C)