TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification
Storage
at -20°C for two years
Shipping
Dry ice (-70°C)
Product Contents
Component |
AP301-01/11 |
AP301-02/12 |
AP301-03/13 |
TransStart® KD Plus DNA Polymerase |
100 U×1 |
200 U×1 |
200 U×6 |
5×TransStart® KD Plus Buffer |
1.2 ml×1 |
1.2 ml ×2 |
1.2 ml ×12 |
2.5 mM dNTPs |
- / 500 μl×1 |
- / 1 ml ×1 |
- / 1 ml ×6 |
50 mM MgSO4 |
200 μl×1 |
400 μl ×1 |
1 ml ×1 |
6×DNA Loading Buffer |
500 μl×1 |
1 ml ×1 |
1 ml ×2 |