This product is a ready-to-use qPCR cocktail. It contains a novel TransStart® TipTaq DNA Polymerase , unique hot start reagents (DNA binding proteins combined with unique chemical), optimized double cation buffer, EvaGreen I, dNTPs, PCR Enhancer, PCR stabilizer and Passive Reference Dye I/II. qPCR SuperMix is provided at 2×concentration and can be used at 1× concentration by adding template, primer and Nuclease-free Water.
Highlights
• A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart® TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
Storage
at -20°C away from light for two years
Shipping
Dry ice (-70°C)
Product Contents
Component |
AQ142-11/21 |
AQ142-12/22 |
AQ142-13/23 |
AQ142-14/24 |
2×TransStart® Tip Green qPCR SuperMix
(+Dye I/+Dye II)
|
1 ml |
5×1 ml |
15×1 ml |
25×1 ml |
Nuclease-free Water |
1 ml |
5 ml |
3×5 ml |
5×5 ml |