TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.
• TransTaq®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent“A”can be generated at the 3’end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.
Applications
• Complex templates
• TA cloning
Storage
at -20°C for two years
Shipping
Dry ice (-70°C)
Product Contents
Component |
AP122-01/11 |
AP122-02/12 |
AP122-03/13 |
TransTaq®-T DNA Polymerase |
250 U×1 |
500 U×1 |
500 U×6 |
10×TransTaq® |
1.2 ml ×1 |
1.2 ml ×1 |
1.2 ml ×6 |
2.5 mM dNTPs |
- / 400 μl ×1 |
- / 800 μl ×1 |
- / 800 μl ×6 |
6×DNA Loading Buffer |
500 μl×1 |
1 ml ×1 |
1 ml ×2 |