TransZol Up utilizes guanidine isothiocyanate to lyse cells. In the process of sample lysis, TransZol Up can maintain the integrity of RNA. After adding RNA ExtractionAgent, the solution is divided into a colorless aqueous phase and a pink organic phase. RNA is in the aqueous phase. RNA can be recovered by precipitation with isopropanol. Isopropyl alcohol recovers protein. Compared with other total RNA extraction reagents, TransZol Up has strong cleavage ability, fast speed, and higher RNA extraction quantity and purity. Suitable for rapid extraction of RNA from a variety of tissues and cells.
Highlights
• High operational safety: RNA Extraction Agent is used instead of chloroform.
• Wide range of applications: animal, plant tissue, blood and bacteria samples. Small samples (50-100 mg tissue, 5 x 106 cells, 200 μl blood). Large samples (≥1 g tissue or ≥107 cells).
• Fast extraction: the reaction can be completed within an hour.
• Visualization of operation: The solution is pink for easy separation of aqueous and organic phases.
• High extraction purity: minimal DNA and protein contamination.
• RNA lysate: facilitates RNA preservation and reduces inhibition of reverse transcription reactions
Storage
at 2-8°C in dark for one year.
Shipping
ice bag 4°C.
Product Contents
Component |
ET111-01 |
TransZol Up |
100 ml |
RNA Extraction Agent |
20 ml |
RNA Dissolving Solution |
15 ml |