TransZol Up Plus RNA Kit is suitable for the isolation of total RNA from cells and tissues. After the sample is lysed with TransZol Up and RNA Extraction Agent is added, the solution separates into an upper colorless aqueous phase (containing RNA), an interphase and a lower pink organic phase. A silica-based spin column is used to specifically bind to RNA in the aqueous phase. Compared with other total RNA extraction methods, TransZol Up Plus RNA Kit possesses not only advantages of strong lysis capability, high yield and a wide range of applications of TransZol Up, but also the advantage of high purity of spin column extraction.
Highlights
• High operational safety: RNA Extraction Agent replaces chloroform.
• A wide range of applications enabled by the maximum binding capacity of the spin column of 100 μg: animal and plant tissues, viruses, bacteria or other samples. Small amount of samples (50-100 mg tissues, 5×106cells, or 200 μl blood) and large amount of samples (≥1g tissues or ≥107cells).
• Strong lysis capability: Complete and fast lysis, high yield.
• Rapid extraction: Completed in one hour.
• Visible workflow: Pink organic phase to facilitate separation from colorless aqueous phase
• High purity: Minimum DNA and protein contamination.
Storage
TransZol Up at 2-8°C away from light for one year, others at room temperature (15°C- 25°C) in a dry place for one year
Shipping
TransZol Up on ice bag (4°C) ; others at room temperature.
Product Contents
Component |
ER501-01 (100 rxns) |
TransZol Up |
100 ml |
RNA Extraction Agent |
20 ml |
Clean Buffer 9 (CB9) |
110 ml |
Wash Buffer 9 (WB9) |
24 ml |
RNase-free Water |
40 ml |
RNase-free Tube (1.5 ml) |
100 |
RNA Spin Column with Collection Tubes |
100 |