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TSA Reagents

TSA Plus Cyanine 3.5

TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.

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Overview

Tyramide Signal Amplification (TSA) is a technology that improves sensitivity by up to 100-fold while allowing reduced consumption of primary antibodies or probes.

Catalog

Catalog #	Product	Size
NEL763001KT	TSA Plus Cyanine 3.5, 50-150 Slides	50-150 Slides

Details

TSA^® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.

TSA Plus kits deliver ultimate sensitivity in immunohistochemistry (IHC), immunofluorescence (IF), and in situ hybridization (ISH) assays. This widely referenced method uses horseradish peroxidase (HRP) to mediate intense covalent deposition of detection labels at very close proximity to the epitope or sequence of interest. TSA Plus has been employed for quantitative detection in a wide variety of sample types including formalin-fixed, paraffin-embedded (FFPE) tissue, cryosections and cultured cells.