SingaporeSG
ChinaCN
MalaysiaMY
IndonesiaID
MyanmarMM
Analytical Science
High Performance Chromatographs (HPLC) / Amino Acid Analyzers (AAA)
Ultra-High Performance Liquid Chromatograph ChromasterUltra Rs
Features
High Resolution
The newly developed LaChromUltra II column is the longest UHPLC column at 250 mm in length. When used together with the reduced volume system ChromasterUltra Rs, it produces an exceptional high-separation performance.
*EachEach column has its own recommended flow rate range for analysis, and it is advisable to abide by these limits in order to maintain and optimize the separation performance of any column.
The combination of this column and the ChromasterUltra Rs enables system pressure tolerance of 140 MPa with 50,000 or more theoretical plates per column.
High Sensitivity
A total-reflection capillary flow cell is incorporated into the diode array detector to answer to the increasing need for high-sensitivity analysis of hazardous substances, genotoxic impurities, etc. A high-sensitivity flow cell with an optical path length of 65 mm is also available. In addition to the capability of high-sensitivity analysis, carryover is reduced through the use of a double-corkscrew mixer.
High-sensitivity total-reflection capillary flow cell
The diode array detector (DAD) exhibits low noise and low drift, achieved through the use of a new optical system providing optimal conditions for high-sensitivity analysis. The optional high-sensitivity flow cell (optical path length of 65 mm) further enhances sensitivity; about a tenfold increase is obtained compared with our previous model (LaChromUltra), thus enabling high-sensitivity applications including impurities from side reactions, genotoxic impurities, etc.
The acquisition of impurity profiles during all stages of synthesis and in raw materials used in medicines and chemicals, intermediate byproducts, and finished drugs are examples of useful applications.
Double-corkscrew mixer
The binary pump is equipped with the latest design in microfluidic double-corkscrew mixers.
An efficient mixing is attained even for a low volume, resulting in a baseline that is extremely stable during gradient analysis, enabling higher-sensitivity analysis.
Structure of the double-corkscrew mixer:
Repeated branching and merging of channels within the mixer provide effective solvent mixing (mixer volume of 55 µL).
[Conditions]
| Column | LaChromUltra II C18(1.9 µm) 2.0 mm I.D.×50 mm L. |
|---|---|
| Eluent | A)0.1% TFA/H2O(v/v) B)0.1% TFA/CH3CN(v/v) A/B=50/50 |
| Flow rate | 0.500 mL/min |
| Detection | UV 214 nm |
Carryover
Low carryover (0.001% or less) is attained via the optimized injection port structure.
- The dead volume of the injection port is reduced
- High-flow-rate washing is achieved through the use of a wash pump dedicated to the autosampler.
- Two solvents are used to wash the inner wall of the needle as well as the inside of the injection valve.
- A back flush function for the injection port is newly incorporated.
Usability
GUI Controller (optional)
All modules can be controlled from the Graphical User Interface (GUI) controller.
The GUI is comprised of a color LCD monitor (5.7-inch color TFT display with LED back-light) and a touch panel made for easy viewing and simple operation.
Up to 10 programs including a timer function, pre-analysis tasks (Wakeup), and post-analysis tasks (Sleep) can be created for a system.
The GUI controller enables you to check the status of consumables usage for all units that are connected to the system.
MEM Column fitting (optional)
Hitachi' s own Moment Enhancing Mechanism (MEM) column fitting is a simple but exciting new development. The finger-tight piping integral fitting, which has a very small dead volume, is capable of safely tolerating system pressures as high as 140 MPa.
MEM column fitting
A view of the operation of attaching a column
Lineup
- Diode array detector (DAD) system
- UV-VIS detector system
Data
Stable solvent delivery allows a board range of analyses from UHPLC to HPLC
Stable solvent delivery is achieved, independent of pressure and solvent composition, by incorporating LBT control and correcting the bulk modulus of the solvent. Stable analyses are supported with 5-µm, 3-µm, and 1.9-µm columns.
Excellent reproducibility
Even for gradient analysis
Under high-pressure conditions, highly reproducible high-resolution results are achieved.
Highly reproducible analyses are also possible for analyses of protein digests, which produce numerous peptide peaks.
High-resolution analysis of related substances (impurities)
Erythromycin (a macrolide antibiotic having a basic structure comprised of a 14-membered ring) is used as a sample model for separation of the main component and related substances to compare the separation using an HPLC column vs. the separation using a UHPLC column.
HPLC column [Conditions]
| Column | LaChrom II C18(5 µm) 4.6 mm I.D.×150 mm L. |
|---|---|
| Flow rate | 1.0 mL/min |
| Eluent | 20 mmol/L Phosphate Buffer/CH3CN/CH3OH=45/40/15 |
| Detection | DAD 210 nm |
UHPLC column [Conditions]
| Column | LaChromUltra II C18(1.9 µm) 3.0 mm I.D.×250 mm L. |
|---|---|
| Flow rate | 0.710 mL/min |
| Eluent | 20 mmol/L Phosphate Buffer/CH3CN/CH3OH=45/40/15 |
| Detection | DAD 210 nm |
More satisfactory separations are attained by the use of the newly developed high resolution column for UHPLC applications.