Overview
Yeast Nitrogen Base media have been prepared according to the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.
Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components. These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.
* Please note all formulations within this product range are based upon Yeast Nitrogen Base without Amino Acids, without Ammonium Sulphate and without the Component/Components selected.
Technical Data
Yeast Morphology Agar
|
Yeast Nitrogen Base
|
Yeast Potassium Nitrate Nitrogen Base
|
Yeast Nitrogen Base w/o Amino Acids
|
Yeast Nitrogen w/o Amino Acids and w/o Ammonium Sulfate
|
Yeast Carbon Base
|
Vitamin Free Yeast Base
|
Nitrogen Source g/l
|
|
|
|
|
|
|
|
Ammonium Sulphate
|
3.5
|
5
|
–
|
5
|
–
|
–
|
5
|
Asparagine
|
1.5
|
–
|
–
|
–
|
–
|
–
|
–
|
Potassium Nitrate
|
–
|
–
|
0.78
|
–
|
–
|
–
|
–
|
Caseine Hydrolysate
|
–
|
–
|
–
|
–
|
–
|
–
|
–
|
Carbon Source g/l
|
|
|
|
|
|
|
|
Glucose.H2O
|
10
|
–
|
–
|
–
|
–
|
10
|
10
|
Galactose
|
–
|
–
|
–
|
–
|
–
|
–
|
–
|
Raffinose
|
–
|
–
|
–
|
–
|
–
|
–
|
–
|
Amino Acids mg/l
|
|
|
|
|
|
|
|
Histidine.HCl
|
10
|
10
|
1
|
–
|
–
|
1
|
10
|
Methionine
|
20
|
20
|
2
|
–
|
–
|
2
|
20
|
Tryptophan
|
20
|
20
|
2
|
–
|
–
|
2
|
20
|
Vitamins µg/l
|
|
|
|
|
|
|
|
Biotin
|
2
|
2
|
2
|
2
|
2
|
2
|
–
|
Ca-Panthotenate
|
400
|
400
|
400
|
400
|
400
|
400
|
–
|
Folic Acid
|
2
|
2
|
2
|
2
|
2
|
2
|
–
|
Inositol
|
2000
|
2000
|
2000
|
2000
|
2000
|
2000
|
–
|
Nicotinic Acid
|
400
|
400
|
400
|
400
|
400
|
400
|
–
|
p-Aminobenzoic Acid
|
200
|
200
|
200
|
200
|
200
|
200
|
–
|
Pyridoxine HCl
|
400
|
400
|
400
|
400
|
400
|
400
|
–
|
Riboflavin
|
200
|
200
|
200
|
200
|
200
|
200
|
–
|
Thiamine HCl
|
400
|
400
|
400
|
400
|
400
|
400
|
–
|
Trace Elements µg/l
|
|
|
|
|
|
|
|
Boric Acid
|
500
|
500
|
500
|
500
|
500
|
500
|
500
|
Copper Sulfate
|
40
|
40
|
40
|
40
|
40
|
40
|
40
|
Potassium Iodide
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
Ferric Chloride
|
200
|
200
|
200
|
200
|
200
|
200
|
200
|
Manganese Sulfate
|
400
|
400
|
400
|
400
|
400
|
400
|
400
|
Sodium Molybdate
|
200
|
200
|
200
|
200
|
200
|
200
|
200
|
Zinc Sulfate
|
400
|
400
|
400
|
400
|
400
|
400
|
400
|
Minerals g/l
|
|
|
|
|
|
|
|
KH2PO4
|
1
|
1
|
1
|
1
|
1
|
1
|
1
|
Magnesium Sulphate.anh
|
0.5
|
0.5
|
0.5
|
0.5
|
0.5
|
0.5
|
0.5
|
Sodium Chloride
|
0.1
|
0.1
|
0.1
|
0.1
|
0.1
|
0.1
|
0.1
|
Calcium Chloride.anh
|
0.1
|
0.1
|
0.1
|
0.1
|
0.1
|
0.1
|
0.1
|
Agar g/l
|
|
|
|
|
|
|
|
Agar
|
18
|
–
|
–
|
–
|
–
|
–
|
–
|
Product Range
- Translucent K+ free medium, YNB w/o Amino acids and without Ammonium sulphate and w/o Potassium and w/o Folic acid and w/o Riboflavin
- Translucent K+ free medium”, YNB w/o Amino acids and without Ammonium sulphate and w/o Potassium
- Yeast Nitrogen Base without Amino acids and without Ammonium sulphate
- Yeast Nitrogen Base without Amino acids, without Ammonium sulphate and without para-Amino Benzoic acid
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Biotin
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Pantothenate
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Copper
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate, without Copper and without Iron
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Folic Acid
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Folic Acid and Riboflavin
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Inositol
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Iodine
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Iron
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Magnesium
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Molybdene
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Nicotinic acid
- Yeast Nitrogen Base without Amino Acids, without Ammonium sulphate and without Phosphate, supplemented with KCl
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Pyridoxine
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Riboflavin
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine
- Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Zinc
For more details, kindly refer to the "Downloads" tab.